Development of a Yeast Bio-Assay to Screen Anti-Mitochondrial Drugs
Johan L.F. Kock,
Chantel W. Swart,
Desmond M. Ncango,
Johan L.F. Kock Jr.,
Ingrid A. Munnik,
Marleen M.J. Maartens,
Carolina H. Pohl,
Pieter W.J. van Wyk.
Previous studies show that acetylsalicylic acid (aspirin) at low concentrations affects yeast sexual structure development in a similar fashion than oxygen depletion. This is ascribed to its anti-mitochondrial action. In this study, we report the same for other anti-inflammatory (i.e. ibuprofen, indomethacin, salicylic acid, benzoic acid) as well as anticancer (Lonidamine) drugs, also known for inhibiting mitochondrial activity in mammalian cells. This is shown by a unique yeast bio-assay, with the mitochondrion-dependent sexual structure, riboflavin production, and hyphal morphology of the yeast Eremothecium ashbyi serving as indicators. These drugs affect this yeast in a similar way as found under oxygen limitation conditions by inhibiting sexual structure development (most sensitive), riboflavin production, and yielding characteristically wrinkled and granular hyphae, presenting a unique “anoxic” morphological pattern for this yeast. Only drugs associated with anti-mitochondrial activity presented such a pattern. This bio-assay may find application in the screening for novel drugs from various sources with anti-mitochondrial actions.
Keywords: Anticancer, antifungal, anti-inflammatory, anti-mitochondrion, bio-assay, yeast
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