Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

Back

Inhibitory Properties of Trapping Agents: Glutathione, Potassium Cyanide, and Methoxylamine, Against Major Human Cytochrome P450 Isoforms

Author(s): Chenghong Zhang, Susan Wong, Erlie M. Delarosa, Jane R. Kenny, Jason S. Halladay, Cornelis E. Hop, Siamak Cyrus Khojasteh-Bakht.

Abstract:

In the early stages of drug discovery, the formation of reactive metabolites is often assessed by co-incubating the drug in liver microsomes with a trapping agent in the presence of NADPH. Our group assessed the capability of commonly used trapping agents to reversibly inhibit major cytochrome P450 (CYP) isoforms. Glutathione and cyanide did not inhibit the enzymes at concentrations up to 10 mM; however methoxylamine did show inhibition, with IC50 values of 0.53 mM for CYP1A2, 4.12 mM for CYP2C9, 2.04 mM for CYP2C19, 9.72 mM for CYP2D6, and 1.26 and > 10 mM for CYP3A4/5 (for testosterone and midazolam, respectively, as substrates).

Keywords: Trapping agents, glutathione, cyanide, methoxylamine, P450 inhibition, reactive metabolites

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

VOLUME: 3
ISSUE: 2
Year: 2009
Page: [125 - 129]
Pages: 5
DOI: 10.2174/187231209788654126
Price: $58