Oxidation Proteomics: The Role of Thiol Modifications
Beat M. Riederer.
Identification of thiol modifications has gained significant importance. It is increasingly recognized that cysteines play an important role in protein function under both physiological and patho-physiological conditions. Here we reviewed different approaches that are used to identify oxidized proteins and discuss different fluorescent labeling techniques, differential two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization – time of flight identification, in short MALDI-TOF. We illuminate processes that depend on protein oxidation of cysteines and we look
Keywords: Aging, cysteine, oxidation, thiol, DIGE, MALDI-TOF, Western blot, dye labeling, disease
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