Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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Oxidation Proteomics: The Role of Thiol Modifications

Author(s): Beat M. Riederer.

Abstract:

Identification of thiol modifications has gained significant importance. It is increasingly recognized that cysteines play an important role in protein function under both physiological and patho-physiological conditions. Here we reviewed different approaches that are used to identify oxidized proteins and discuss different fluorescent labeling techniques, differential two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization – time of flight identification, in short MALDI-TOF. We illuminate processes that depend on protein oxidation of cysteines and we look

Keywords: Aging, cysteine, oxidation, thiol, DIGE, MALDI-TOF, Western blot, dye labeling, disease

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Article Details

VOLUME: 6
ISSUE: 1
Year: 2009
Page: [51 - 62]
Pages: 12
DOI: 10.2174/157016409787847448