Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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Recent Developments in Mass Spectrometry Analysis of Phosphoproteomes

Author(s): Chhabil Dass.

Abstract:

Phosphorylation is one of the most important and ubiquitous modifications in eukaryotic cells. This covalent modification is a major signaling pathway in living beings. A vast array of cellular events, such as proliferation, differentiation, metabolism, signal transduction, and adaptation to environmental stress, and the function of many proteins, hormones, neurotransmitters, and enzymes, are triggered by phosphorylation. For understanding highly interconnected regulatory network, it is essential to identify and quantify phosphoproteins in biological specimens. Currently, this task is accomplished by mass spectrometry-driven phosphoproteomics. This article outlines recent developments in the analysis of phosphoproteins, specifically, the enrichment, detection, identification, and quantification of phosphopeptides/phosphoproteins.

Keywords: Mass spectrometry, phosphorylation, phosphopeptides, phosphoproteins, phosphoproteomics, shotgun proteomics, quantitative phosphoproteomics, enrichment of phosphopeptides

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Article Details

VOLUME: 6
ISSUE: 1
Year: 2009
Page: [32 - 42]
Pages: 11
DOI: 10.2174/157016409787847394