Selective Gene Amplification for High-Throughput Sequencing
Ting Ni, Han Wu, Shen Song, Mark Jelley and Jun Zhu
Affiliation: Institute for Genome Sciences&Policy, Department of Cell Biology, Duke University Medical Center, 101 Science Drive, Durham, NC 27708, USA.
Keywords: Gene-collector, selector, megaPlex PCR, multiplex exon capture, sequence-capture array, selective amplification, nextGen sequencing, deep sequencing
With tremendous progress in next generation sequencing technologies, it has become possible to perform genomic sequencing in a highly cost-effective manner. However, the human genome remains too complex to be routinely re-sequenced. Thus, selective gene amplification is often employed to target a subset of genomic regions (such as a set of disease-related genes) for deep sequencing analysis. Herein, we briefly review patents that can enrich target genomic regions of interest to harness the power of NextGen sequencing technology for basic and translational biomedical research.
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