Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

Back

Functional Glyco-Affinity Precipitation/Capturing for Enhanced Affinity Proteomics

Author(s): Xue-Long Sun.

Abstract:

Proteomics research typically starts by reducing the sample complexity through multidimensional separation methods based on the unique characteristics of the proteins or their peptides followed by identification and quantification. Protein-carbohydrate bindings play very important roles in cell-cell communication, cell proliferation and differentiation as well as microbial adhesion. Therefore, carbohydrates can serve as protein capturing ligand for protein purification and identification and protein-carbohydrate binding research provides important insights for basic biological research, medicine and the biotechnology as well as drug development. In the past decades, a diverse carbohydrate-based technology, termed glycotechnology, such as glyco-arrays and glyco-affinity chromatography, and glyco-affinity probe, have been developed. A guiding principle of these glycotechnologies is the display of carbohydrates onto a pertinent support or carrier such as glass slide, microplate, and microbeads as well as functional polymers and probe molecules. This review highlights recent progresses of carbohydrate-containing intelligent polymers for rapid and efficient glyco-affinity precipitation purification of carbohydrate-binding protein and the enhanced proteomics research. Specifically, two innovation methods, inverse reversible thermal-responsive glyco-affinity precipitation and magnetic assistant glyco-affinity precipitation purification of carbohydrate-binding protein for enhanced proteomics application are summarized.

Keywords: Carbohydrate, protein, glyco-affinity, stimulus responsive, polymer, precipitation, proteomics

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

VOLUME: 5
ISSUE: 3
Year: 2008
Page: [202 - 210]
Pages: 9
DOI: 10.2174/157016408785909640
Price: $58