Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics
Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura and Hiroyuki Imanaka
Affiliation: Div. of Chem. and Biochem., Grad. School of Natural Sci. and Technol., Okayama Univ., 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.
Keywords: Controlled immobilization, site-directed immobilization, proteome analysis, affinity peptide, ELISA, proteinprotein interaction
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