Effects of Enzyme Sources on Midazolam 1-Hydroxylation Activity Catalyzed by Recombinant Cytochrome P450 3A4 in Combination with NADPH-Cytochrome P450 Reductase

Author(s): Chie Emoto, Norie Murayama, Hiroshi Yamazaki.

Journal Name: Drug Metabolism Letters

Volume 2 , Issue 3 , 2008

Become EABM
Become Reviewer


The influence of the enzyme source such as phospholipid and expression ratio of NADPHcytochrome P450 reductase on midazolam 1-hydroxylation activity of cytochrome P450 3A4 was investigated in reconstituted systems and in membranes using bicistronic expressions. Preferable ratio of P450 reductase over human P450 3A4 would be a determinant factor for the drug metabolism studies.

Keywords: CYP3A4, lipid mixtures, NADPH-P450 reductase, bicistronic expression, E. coli membranes, human

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2008
Page: [190 - 192]
Pages: 3
DOI: 10.2174/187231208785425737
Price: $58

Article Metrics

PDF: 4