Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Effects of Enzyme Sources on Midazolam 1-Hydroxylation Activity Catalyzed by Recombinant Cytochrome P450 3A4 in Combination with NADPH-Cytochrome P450 Reductase

Author(s): Chie Emoto, Norie Murayama, Hiroshi Yamazaki.

Abstract:

The influence of the enzyme source such as phospholipid and expression ratio of NADPHcytochrome P450 reductase on midazolam 1-hydroxylation activity of cytochrome P450 3A4 was investigated in reconstituted systems and in membranes using bicistronic expressions. Preferable ratio of P450 reductase over human P450 3A4 would be a determinant factor for the drug metabolism studies.

Keywords: CYP3A4, lipid mixtures, NADPH-P450 reductase, bicistronic expression, E. coli membranes, human

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Article Details

VOLUME: 2
ISSUE: 3
Year: 2008
Page: [190 - 192]
Pages: 3
DOI: 10.2174/187231208785425737
Price: $58