Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Time and Dose Dependent Study of Doxorubicin Induced DU-145 Cytotoxicity

Author(s): Saeed S. Al-Ghamdi.

Abstract:

Doxorubicin is one of the most effective anti-tumour agent. To clarify whether a single dose produces its effects soon after dosing or only after hours or days and whether the effects are brief or prolonged, time-dose relationships were explored by calculating lethal time (LT50) values which is a statistical estimate of the time from dosage to death of 50% of the organisms/ or cells in a very large population subjected to a toxicant under specific conditions. This was achieved by the log-time log-dose curve which may be used to predict the proper doses to be used in long-term studies. Drug chemosensitivity using DU-145 cell lines have been investigated for this purpose. The results shows that the effects of doxorubicin started only after 24hrs; however, resistance was developed, 40 hrs was the time required to kill 50% of cells , and post-incubation with fresh media (F.M.) exhibited more cell damages. It is concluded that doxorubicin is effective only after 24 hrs with resistance developed and post-incubation with F.M after treating cells with doxorubicin causes more damage than continuous incubation with the drug.

Keywords: Doxorubicin, DU-145, cytotoxicity

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Article Details

VOLUME: 2
ISSUE: 1
Year: 2008
Page: [47 - 50]
Pages: 4
DOI: 10.2174/187231208783478443