Rapid and Sensitive Characterization of the Metabolite Formation Enzyme Kinetics of Radiolabeled Drugs Using Stop-Flow Liquid Radiochromatography

Title: Rapid and Sensitive Characterization of the Metabolite Formation Enzyme Kinetics of Radiolabeled Drugs Using Stop-Flow Liquid Radiochromatography

Volume: 2 Issue: 1

Author(s): Lifei Wang, Weiping Zhao, Donglu Zhang and Mingshe Zhu

Affiliation:

Keywords: Stop-flow RFD, enzyme kinetics, liquid radiochromatography

Abstract: This study evaluated the reproducibility, sensitivity, accuracy, and precision of stop-flow liquid radiochromatographic detection (RFD). Stop-flow RFD was about 10-fold more sensitive compared with traditional RFD and had a good reproducibility for quantification and HPLC retention time. Stop-flow RFD was applied to determine enzyme kinetics of radiolabeled drugs. The enzyme kinetic parameters of muraglitazar glucuronidation determined by stop-flow RFD were comparable with those determined by microplate scintillation counting (MSC).

Cite this article as:

Wang Lifei, Zhao Weiping, Zhang Donglu and Zhu Mingshe, Rapid and Sensitive Characterization of the Metabolite Formation Enzyme Kinetics of Radiolabeled Drugs Using Stop-Flow Liquid Radiochromatography, Drug Metabolism Letters 2008; 2 (1) . https://dx.doi.org/10.2174/187231208783478470