Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Rapid and Sensitive Characterization of the Metabolite Formation Enzyme Kinetics of Radiolabeled Drugs Using Stop-Flow Liquid Radiochromatography

Author(s): Lifei Wang, Weiping Zhao, Donglu Zhang, Mingshe Zhu.

Abstract:

This study evaluated the reproducibility, sensitivity, accuracy, and precision of stop-flow liquid radiochromatographic detection (RFD). Stop-flow RFD was about 10-fold more sensitive compared with traditional RFD and had a good reproducibility for quantification and HPLC retention time. Stop-flow RFD was applied to determine enzyme kinetics of radiolabeled drugs. The enzyme kinetic parameters of muraglitazar glucuronidation determined by stop-flow RFD were comparable with those determined by microplate scintillation counting (MSC).

Keywords: Stop-flow RFD, enzyme kinetics, liquid radiochromatography

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Article Details

VOLUME: 2
ISSUE: 1
Year: 2008
Page: [41 - 46]
Pages: 6
DOI: 10.2174/187231208783478470