Real-Time Polymerase Chain Reaction Detection Methods
Douglas F. Whitman and Sherry A. Dunbar
Affiliation: Luminex Corporation, 12212 Technology Blvd., Austin, TX 78727, USA.
Keywords: Real-time PCR, Kinetic PCR, Quantitative PCR, hybridization probes, TaqMan®, FRET probes, hairpin probes, molecular beacon, SYBR® Green, hydrolysis probes
Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.
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