Real-Time Polymerase Chain Reaction Detection Methods

Author(s): Douglas F. Whitman, Sherry A. Dunbar.

Journal Name:Recent Patents on DNA & Gene Sequences (DIscontinued)

Volume 2 , Issue 1 , 2008


Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.

Keywords: Real-time PCR, Kinetic PCR, Quantitative PCR, hybridization probes, TaqMan®, FRET probes, hairpin probes, molecular beacon, SYBR® Green, hydrolysis probes

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Article Details

Year: 2008
Page: [20 - 26]
Pages: 7
DOI: 10.2174/187221508783406558