Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

Back

Mass Defect Filtering on High Resolution LC/MS Data as a Methodology for Detecting Metabolites with Unpredictable Structures: Identification of Oxazole-Ring Opened Metabolites of Muraglitazar

Author(s): Donglu Zhang, Peter T. Cheng, Haiying Zhang.

Abstract:

This study describes the application of the mass defect filter method for the detection of two unpredicted oxazole- ring opened metabolites of muraglitazar in the feces of humans following oral administration. Unlike other muraglitazar metabolites, these metabolites formed little to no protonated ions, and the NH4 + or Na+ adduct ions that were formed were weak and not discernible from fecal interferences even after background subtraction. With mass defect filtering on high resolution LC/MS data, the resulting total ion chromatogram and the simplified mass spectra allowed for the identification and characterization of these metabolite ions, and their structures were confirmed by synthesis.

Keywords: Mass defect filter, metabolite detection, metabolite profiling, Muraglitazar

Order Reprints Order Eprints Rights & PermissionsPrintExport

Article Details

VOLUME: 1
ISSUE: 4
Year: 2007
Page: [287 - 292]
Pages: 6
DOI: 10.2174/187231207783221466
Price: $58