Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Comprehensive Identification of Cytochrome P450 Isoforms from Solubility-Based Fractionated Rat Liver Microsomes

Author(s): Hidenori Yamanaka, Masahiro Takeyoshi, Yasushi Minobe, Yoshikuni Yakabe, Mineo Takatsuki, Hisaya Sato.

Abstract:

Cytochrome P450 isoforms from male rat liver microsomes were comprehensively identified using nano liquid chromatography tandem mass spectrometry (nanoLC-MS/MS). The enrichment of P450, an endomembrane-anchored heme protein, was achieved by solubility-based protein fractionation, and greatly improved the total number of identified P450 isoforms. LC-MS/MS analysis of fractions resulted in the identification of total 36 P450 isoforms. The combination of proteomic analysis and the solubility-based fractionation would provide powerful tool for the expression analysis of the superfamily proteins having great similarities between the amino acids sequences.

Keywords: Drug metabolism, membrane proteins, P450 isoforms, rat liver microsome

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Article Details

VOLUME: 1
ISSUE: 4
Year: 2007
Page: [281 - 286]
Pages: 6
DOI: 10.2174/187231207783221394
Price: $58