Metabolite Interference in Pharmacokinetic Studies
Mehran F. Moghaddam,
Jason C. Katz,
Michael A. Shirley.
Our policy of conducting biotransformation studies with extended chromatography prior to pharmacokinetic bioanalyses allowed us to quickly detect an unusual, cis/trans metabolite in rat plasma that was inseparable using a short chromatographic method. We caution investigators that short methods invite unknown isobaric metabolites to cause inaccuracies in plasma concentration measurements.
Keywords: Pharmacokinetics, metabolite, isomer, chromatography, coelution, aminocyclohexanol
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