Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Metabolite Interference in Pharmacokinetic Studies

Author(s): Mehran F. Moghaddam, Jui-Hsiang Fan, Yang Tang, Oleg Khatsenko, Jason C. Katz, Michael A. Shirley.

Abstract:

Our policy of conducting biotransformation studies with extended chromatography prior to pharmacokinetic bioanalyses allowed us to quickly detect an unusual, cis/trans metabolite in rat plasma that was inseparable using a short chromatographic method. We caution investigators that short methods invite unknown isobaric metabolites to cause inaccuracies in plasma concentration measurements.

Keywords: Pharmacokinetics, metabolite, isomer, chromatography, coelution, aminocyclohexanol

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Article Details

VOLUME: 1
ISSUE: 4
Year: 2007
Page: [272 - 275]
Pages: 4
DOI: 10.2174/187231207783221475
Price: $58