Current Proteomics

Bernd Rehm  
Institute of Molecular BioSciences Massey University
Private Bag 11222
Palmerston North
New Zealand

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ELISA-based Protein Arrays: Multiplexed Sandwich Immunoassays

Author(s): Ruo-Pan Huang, Ying Lin, Li-Pai Chen, Weimin Yang, Ruochun Huang.

Abstract:

Protein arrays with ability to simultaneously detect multiple protein expression levels, protein-protein interactions, protein-DNA or RNA interactions, protein-small molecule interactions and protein modifications will be an excellent tool in biomedical discovery. A variety of technologies have been developed to meet this need. In general, these assay systems can be divided into two major categories: label-based approaches, and sandwich or enzyme linked immunosorbent assay (ELISA)-based approaches. The purpose of this review is to provide an overview of the principles and techniques of ELISA-based protein arrays, with particular emphasis on the use of ELISA-based protein arrays for detection of multiple protein expression levels.

Keywords: protein arrays, antibody arrays, proteomics, elisa, protein expression, immunoassay, cytokine

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Article Details

VOLUME: 1
ISSUE: 3
Year: 2004
Page: [199 - 210]
Pages: 12
DOI: 10.2174/1570164043379226
Price: $58