Studies of Bioactivity, Conformation and Pharmacokinetic Profiles of Site-Specific PEGylated Thymosin Alpha 1 Derivatives
Site-specific mono-PEGylations were performed in different conformational regions of Thymosin alpha 1 (Tα1) by introducing one cysteine residue into the chosen site and coupling with thiol-specific mPEG-MAL reagent. Results demonstrated that PEGylated sites and regions influenced the conformations and pharmacokinetic profiles of the peptide greatly with following order: α-helix, β-turn, random coil and terminals, but little on the immunoactivity.
Keywords: Site-specific PEGylation, thymosin alpha 1, immunoactivity
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