The Utility of Stable Cell Lines to Assess Species Differences in PXR Transactivation
Michael W. Sinz,
A stable cell line was developed to assess activation of rat pregnane X receptor (rPXR) by xenobiotics. Characterization revealed that the greatest response occurred with dexamethasone (6-10 fold increase) while rifampicin failed to transactivate rPXR. Rodent PXR transactivation assays can be used to understand species differences in enzyme induction.
Keywords: Rat PXR, stable cells, CYP3A induction, toxicity, high volume screening
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