Drug Metabolism Letters

Zhiyang Zhao
Amgen
Cambridge, MA
USA

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Assessment of Cryopreserved Hepatocytes as an Alternative to Fresh Hepatocytes forComparative InterspeciesMetabolism Studieswith Suitable Acceptance Criteria

Author(s): Ziqiang (Zack) Cheng, Christine E. Herron, Gary Bowers, Mark de Serres.

Abstract:

Fresh hepatocytes have been the choice for interspecies comparative drug metabolism studies. Cryopreserved hepatocytes represent a readily available alternative when combined with acceptance limits based on the metabolic turnover of 7-ethoxycoumarin. Results for the ten NCEs examined show that the metabolites formed were strongly correlated in fresh and cryopreserved hepatocytes.

Keywords: Cryopreserved hepatocytes, fresh hepatocytes, comparative metabolism, metabolite profiling, structural identification, radiochemical flow detection

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Article Details

VOLUME: 1
ISSUE: 2
Year: 2007
Page: [109 - 120]
Pages: 12
DOI: 10.2174/187231207780363624
Price: $58