Assessment of Cryopreserved Hepatocytes as an Alternative to Fresh Hepatocytes forComparative InterspeciesMetabolism Studieswith Suitable Acceptance Criteria
Ziqiang (Zack) Cheng,
Christine E. Herron,
Mark de Serres.
Fresh hepatocytes have been the choice for interspecies comparative drug metabolism studies. Cryopreserved hepatocytes represent a readily available alternative when combined with acceptance limits based on the metabolic turnover of 7-ethoxycoumarin. Results for the ten NCEs examined show that the metabolites formed were strongly correlated in fresh and cryopreserved hepatocytes.
Keywords: Cryopreserved hepatocytes, fresh hepatocytes, comparative metabolism, metabolite profiling, structural identification, radiochemical flow detection
Rights & PermissionsPrintExport