Separation and Identification of Antibacterial Chamomile Components Using OPLC, Bioautography and GC-MS

Author(s): Agnes M. Moricz, Szabolcs Szarka, Peter G. Ott, Eva B. Hethelyi, Eva Szoke, Erno Tyihak.

Journal Name: Medicinal Chemistry

Volume 8 , Issue 1 , 2012

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Abstract:

Components of 50% aqueous ethanol chamomile (Matricaria recutica L.) flower extract, previously found antibacterial in a TLC-bioautographic study, were separated and isolated by the use of on-line overpressured layer chromatography (OPLC). This system consisted of an OPLC 50 BS system, an on-line coupled flow-through UV detector, and a manual fraction collector. The collected fractions were investigated by GC-MS analysis and by TLC re-chromatography with subsequent visualization, performed after use of the vanillin-sulphuric acid reagent, or under UV illumination, or applying bioautographic detection. The main compounds of the collected 11 fractions were identified by GC-MS. The results showed that the antibacterial effect of 50% aqueous ethanol extract of chamomile is ascribable to cis-, trans-spiroethers, and the coumarins like herniarin and umbelliferone.

Keywords: Antibacterial activity, bioautography, chamomile, coumarins, GC-MS, Matricaria recutita L, OPLC, spiroethers, oils, Gram-positive bacteria

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Article Details

VOLUME: 8
ISSUE: 1
Year: 2012
Page: [85 - 94]
Pages: 10
DOI: 10.2174/157340612799278487

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