Prostaglandin E2 Binding Peptide: a Potential Modulatory Agent which Acts Through Suppressing NF-kappaB Signaling in RA

Title: Prostaglandin E2 Binding Peptide: a Potential Modulatory Agent which Acts Through Suppressing NF-kappaB Signaling in RA

Volume: 19 Issue: 2

Author(s): Dongmei Yan, Weihai Peng, Xiangfeng Zhao, Xiao Han, Qihui Liu, Peng Li, Bairong Du and Xun Zhu

Affiliation:

Keywords: Cytokine, inflammation, modulator, NF-κB, PGE2 binding peptide, rheumatoid arthritis, cyclooxygenase-2 (COX-2), interleukin (IL)-1, cytokines, EP4 receptor

Abstract: In an earlier study, we found PBP inhibited the progress of adjuvant-induced arthritis (AA). This study was aimed at evaluating the inhibitory effects of PBP in terms of NF-κB activation by using immunohistochemical and immunofluorescent technique in vitro and in vivo. IL-1β and TNF-α in serum were detected by method of ELISA. Immunofluorescent results showed that PBP inhibited NF-κB p65 translocation into nucleus. In vivo imaging showed that treatment with PBP decreased the enzyme labeling signal of NF-κB p65. Immunohistochemical staining revealed that PBP suppressed production of NF-κB p65 subunit in the joints and attenuated the productions of IL-1β and TNF-α in serum from AA. Moreover, NF-κB p65 nucleus translocation was prevented by simultaneous incubation with PBP and PGE2 was decreased by PBP through a feedback cycle. We report the first confirmation of the mimotope of PGE2 receptor EP4 modulatory action.

Cite this article as:

Yan Dongmei, Peng Weihai, Zhao Xiangfeng, Han Xiao, Liu Qihui, Li Peng, Du Bairong and Zhu Xun, Prostaglandin E2 Binding Peptide: a Potential Modulatory Agent which Acts Through Suppressing NF-kappaB Signaling in RA, Protein & Peptide Letters 2012; 19 (2) . https://dx.doi.org/10.2174/092986612799080329