Prostaglandin E2 Binding Peptide: a Potential Modulatory Agent which Acts Through Suppressing NF-kappaB Signaling in RA

Author(s): Dongmei Yan, Weihai Peng, Xiangfeng Zhao, Xiao Han, Qihui Liu, Peng Li, Bairong Du, Xun Zhu.

Journal Name: Protein & Peptide Letters

Volume 19 , Issue 2 , 2012

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Abstract:

In an earlier study, we found PBP inhibited the progress of adjuvant-induced arthritis (AA). This study was aimed at evaluating the inhibitory effects of PBP in terms of NF-κB activation by using immunohistochemical and immunofluorescent technique in vitro and in vivo. IL-1β and TNF-α in serum were detected by method of ELISA. Immunofluorescent results showed that PBP inhibited NF-κB p65 translocation into nucleus. In vivo imaging showed that treatment with PBP decreased the enzyme labeling signal of NF-κB p65. Immunohistochemical staining revealed that PBP suppressed production of NF-κB p65 subunit in the joints and attenuated the productions of IL-1β and TNF-α in serum from AA. Moreover, NF-κB p65 nucleus translocation was prevented by simultaneous incubation with PBP and PGE2 was decreased by PBP through a feedback cycle. We report the first confirmation of the mimotope of PGE2 receptor EP4 modulatory action.

Keywords: Cytokine, inflammation, modulator, NF-κB, PGE2 binding peptide, rheumatoid arthritis, cyclooxygenase-2 (COX-2), interleukin (IL)-1, cytokines, EP4 receptor

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Article Details

VOLUME: 19
ISSUE: 2
Year: 2012
Page: [212 - 218]
Pages: 7
DOI: 10.2174/092986612799080329
Price: $58

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