Activation of Large Form Galanin-LI by Extracellular Processing in Small Cell Lung Carcinoma Tissue

Author(s): Hiroyuki Yamamoto, Kazuaki Iguchi, Satoshi Ohno, Takashi Yokogawa, Kazuya Nishikawa, Minoru Hoshino.

Journal Name: Protein & Peptide Letters

Volume 18 , Issue 10 , 2011

Abstract:

Galanin is a neuropeptide that is widely distributed in the central and peripheral nervous systems. Some small cell lung carcinoma (SCLC) cell lines such as SBC-3A release only the high-molecular-mass form, with lower molecular mass forms being undetectable. To investigate the mechanism of processing of progalanin to active peptide, we studied galanin-LI in both the culture media of SBC-3A cells and in extracts from in vivo mouse SBC-3A tumors. SBC-3A cells were found to release high molecular mass galanin, but did not release active peptides. In contrast, tumor extract contained both high-molecular-mass galanin, and a cleaved lower-molecular-mass form of the peptide (8, 5 and 2 kDa). The lower-molecular-mass peptide was identified as galanin(1-20) by MALDI-TOF mass spectrometry. We then looked at MMP-2 and MMP-9 release from SBC-3A cells and tumor tissue treated with galanin and progalanin, as revealed by gelatin zymography. Galanin elicited pro-MMP-2 and pro-MMP-9 release from SBC-3A cells and tumor tissue; however, recombinant progalanin induced pro-MMP-2 and pro-MMP-9 release from tumor tissue only. This study has shown that the galanin-LI released from SCLC SBC-3A cells consisted of the high-molecular-mass peptide form, and was processed extracellularly to galanin(1-20). Furthermore, galanin was seen to induce pro-MMP-2 and pro-MMP-9 release from SBC-3A cells.

Keywords: Neuropeptide, extracellular-processing, galanin, tumor, matrix metalloproteinase, propeptide, pro-MMP-2, SCLC, MALDI-TOF, galanin-LI, progalanin, GMAP, ERK, FBSNeuropeptide, extracellular-processing, galanin, tumor, matrix metalloproteinase, propeptide, pro-MMP-2, SCLC, MALDI-TOF, galanin-LI, progalanin, GMAP, ERK, FBS

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Article Details

VOLUME: 18
ISSUE: 10
Year: 2011
Page: [1058 - 1064]
Pages: 7
DOI: 10.2174/092986611796378693
Price: $58

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