Gene Cloning and Homology Modeling of the 3-Oxoacyl-ACP Synthase from Aeromonas hydrophila for Drug Discovery

Vijai      Singh; Indra      Mani; Dharmendra      Kumar Chaudhary

Abstract

Fatty acid biosynthesis in the Aeromonas hydrophila plays an important role in the formation of cell membrane and cell viability. The 3-oxoacyl- acyl carrier protein (ACP) synthase fadH and fadB from A. hydrophila have contributed in the initiation and elongation of fatty acid biosynthesis. In this study, we have designed new set of primers for amplification of the open reading frame of fadH and fadB. Cloning and sequencing of these amplified genes, analyzed that the G+C content of fadH and fadB were 63.3% and 68.79% respectively. The homology modeling was performed to generate the 3- D structure of fadH and fadB; and showed that more than 90% amino acid residues in allowed region of Ramachandranan plot. These findings provide a new avenue for better understanding, the role of genes encoding proteins involved in the fatty acid biosynthesis and thereby using as a potential and novel target for structure based drug designing to control the infections of A. hydrophila.

Keywords: Aeromonas hydrophila, Drugs, fadH, fadB, Fatty acid, Homology modeling, Ramachandranan plot, Fatty acid biosynthesis, amplified genes, Gram-negative bacteria, nuclear magnetic resonance (NMR), Aquaculture, Pseudemis scripta, meningitis, immunocompromised human