Crystallization and Preliminary X-Ray Crystallographic Analysis of CTXM- 15, an Extended-spectrum β-Lactamase Conferring Worldwide Emerging Antibiotic Resistance

Author(s): Young Jun An, Jung Hun Lee, Ha Il Jung, Seung Ghyu Sohn, Jae Jin Lee, Kwang Seung Park, Xing Wu, Byeong Chul Jeong, Choong-Min Kang, Sun-Shin Cha, Sang Hee Lee.

Journal Name: Protein & Peptide Letters

Volume 18 , Issue 9 , 2011

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Abstract:

CTX-M-15, an extended-spectrum β-lactamase emerging worldwide, hydrolyzes lactam ring of β-lactam antibiotics, and thus causes therapeutic failure and a lack of eradication of pathogenic bacteria by third-generation β-lactams. Therefore, the enzyme is a potential target for developing agents against pathogens isolated from patients suffering from nosocomial infections. The CTX-M-15 protein was purified and crystallized at 298 K. X-ray diffraction data from CTXM- 15 crystal have been collected to 1.46 Å resolution using synchrotron radiation. The crystal of CTX-M-15 belongs to space group P212121, with unit-cell parameters a = 45.50, b = 44.23, and c = 116.92 Å. Analysis of the packing density shows that the asymmetric unit probably contains two molecules with a solvent content of 41.26%.

Keywords: Cephalosporins, variants, oxyimino β-lactams, ESBLs, antibiotics, polyacrylamide SDS gel, Polyethylene glycol, Polymerase chain reaction, Asp240Gly mutation, liquid nitrogen, Escherichia coli, synchrotron radiation, nosocomial infections, third-generation β-lactams, lactam ring, extended-spectrum β-lactamase, CTX-M-15, crystal, ceftazidime, cefotaxime, Antibiotic resistanceCephalosporins, variants, oxyimino β-lactams, ESBLs, antibiotics, polyacrylamide SDS gel, Polyethylene glycol, Polymerase chain reaction, Asp240Gly mutation, liquid nitrogen, Escherichia coli, synchrotron radiation, nosocomial infections, third-generation β-lactams, lactam ring, extended-spectrum β-lactamase, CTX-M-15, crystal, ceftazidime, cefotaxime, Antibiotic resistance

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Article Details

VOLUME: 18
ISSUE: 9
Year: 2011
Page: [858 - 862]
Pages: 5
DOI: 10.2174/092986611796011400
Price: $58

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