Abstract
The protein translocations across mitochondrial membranes are carried out by specialized complexes, the Translocase of Outer Membrane (TOM) and Translocase of Inner Membrane (TIM). TIM23 translocon is responsible for translocating the mitochondrial matrix proteins across the mitochondrial inner membrane. Tim44 is an essential, peripheral membrane protein in TIM23 complex. Tim44 is tightly associated with the inner mitochondrial membrane on the matrix side. The Tim44 C-Terminal Domain (CTD) functions as an Inner Mitochondrial Membrane (IMM) anchor that recruits the Presequence protein Associated Motor (PAM) to the TIM23 channel. Using X-ray crystallographic and biochemical data, we show that the N-terminal helices A1 and A2 of Tim44 – CTD are crucial for its membrane tethering function. Based on our data, we propose a model showing how the N-terminal A1 and A2 amphipathic helices can either expose their hydrophobic face during membrane binding or conceal it in the soluble form. Therefore, the A1 and A2 helices of Tim44 may function as a membrane sensor.
Keywords: Mitochondria, peripheral membrane protein, translocation, TIM23, Tim44Mitochondria, peripheral membrane protein, translocation, TIM23, Tim44
Protein & Peptide Letters
Title: Membrane Binding Mechanism of Yeast Mitochondrial Peripheral Membrane Protzein TIM44
Volume: 18 Issue: 7
Author(s): Wenjun Cui, Ratnakar Josyula, Jingzhi Li, Zhengqing Fu and Bingdong Sha
Affiliation:
Keywords: Mitochondria, peripheral membrane protein, translocation, TIM23, Tim44Mitochondria, peripheral membrane protein, translocation, TIM23, Tim44
Abstract: The protein translocations across mitochondrial membranes are carried out by specialized complexes, the Translocase of Outer Membrane (TOM) and Translocase of Inner Membrane (TIM). TIM23 translocon is responsible for translocating the mitochondrial matrix proteins across the mitochondrial inner membrane. Tim44 is an essential, peripheral membrane protein in TIM23 complex. Tim44 is tightly associated with the inner mitochondrial membrane on the matrix side. The Tim44 C-Terminal Domain (CTD) functions as an Inner Mitochondrial Membrane (IMM) anchor that recruits the Presequence protein Associated Motor (PAM) to the TIM23 channel. Using X-ray crystallographic and biochemical data, we show that the N-terminal helices A1 and A2 of Tim44 – CTD are crucial for its membrane tethering function. Based on our data, we propose a model showing how the N-terminal A1 and A2 amphipathic helices can either expose their hydrophobic face during membrane binding or conceal it in the soluble form. Therefore, the A1 and A2 helices of Tim44 may function as a membrane sensor.
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Cite this article as:
Cui Wenjun, Josyula Ratnakar, Li Jingzhi, Fu Zhengqing and Sha Bingdong, Membrane Binding Mechanism of Yeast Mitochondrial Peripheral Membrane Protzein TIM44, Protein & Peptide Letters 2011; 18 (7) . https://dx.doi.org/10.2174/092986611795445996
DOI https://dx.doi.org/10.2174/092986611795445996 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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