Induction of β-1,3-Glucanase in Seeds of Maize Defective-Kernel Mutant (827Kpro1)
Alan Trindade Branco, Beatriz dos Santos Ferreira, Guinevere Fernandes Lourenco, Valeria Cristina Lopes Marques, Olga Lima Tavares Machado, Messias Gonzaga Pereira, Joao Carlos Aquino Almeida and Gonçalo Apolinario de Souza Filho
Affiliation: Universidade Estadual do Norte Fluminense Darcy Ribeiro - Centro de Biociencias e Biotecnologia - Laboratorio de Biotecnologia - Av. Alberto Lamego, 2000 - Parque California - Campos dos Goytacazes - CEP 28013-602 - R.J. - Brazil
Keywords: β-1, 3-glucanase, defective kernel, dek, maize, seed, seed developmentβ-1, 3-glucanase, defective kernel, dek, maize, seed, seed development
β-1,3-glucanases are found in organisms as diverse as plants, animals, bacteria and fungi. In plants, such enzymes are not only associated with defense mechanisms against pathogens, but also play critical roles in physiological and developmental processes. Here we identified a new β-1,3-glucanase in maize seeds, and named it ZmGlucA. Sequence analysis revealed that ZmGlucA belongs to the class A of β-1,3-glucanase, a class related to defense and physiological processes in plants. mRNA and protein assays showed that zmGlucA is expressed exclusively in seeds, and it is differentially regulated during seed development. Additionally, zmGlucA expression is strongly induced in seeds of the mutant dek 827Kpro1, which is defective for embryo and endosperm development. Our data support the idea that ZmGlucA protein is relevant to seed development.
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