Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
USA
Email: bdunn@ufl.edu

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Purification and Partial Characterization of a New Pro-Inflammatory Lectin from Bauhinia bauhinioides Mart (Caesalpinoideae) Seeds

Author(s): Helton C. Silva, Alfa U. Bari, Francisco N. Pereira-Junior, Rafael C. Simoes, Ito L. Barroso-Neto, Camila B. Nobre, Maria G. Pereira, Kyria S. Nascimento, Bruno Anderson M. Rocha, Plinio Delatorre, Celso S. Nagano, Ana Maria S. Assreuy, Benildo S. Cavada.

Abstract:

A new galactose-specific lectin, named BBL, was purified from seeds of Bauhinia bauhinioides by precipitation with ammonium sulfate, followed by two steps of ion exchange chromatography. BBL haemagglutinated rabbit erythrocytes (native and treated with proteolytic enzymes) showing stability even after exposure to 60 °C for one hour. The lectin haemagglutinating activity was optimum between pH 8.0 and 9.0 and inhibited after incubation with D-galactose and its derivatives, especially α-methyl-D-galactopyranoside. The pure protein possessed a molecular mass of 31 kDa by SDS – PAGE and 28.310 Da by mass spectrometry. The lectin pro-inflammatory activity was also evaluated. The s.c. injection of BBL into rats induced a dose-dependent paw edema, an effect that occurred via carbohydrate site interaction and was significantly reduced by L-NAME, suggesting an important participation of nitric oxide in the late phase of the edema. These findings indicate that BBL can be used as a tool to better understand the mechanisms involved in inflammatory responses.

Keywords: Lectin, bauhinia bauhinioides, caesalpinoideae, pro-inflammatory activity, galactose-specific lectin, proteolytic enzymes, L-NAME, Lectin Purification, Molecular Mass Determination, Electrospray ionization, mass spectrometry (ESI-MS), bovine serum albumin (BSA), Rat Paw Edema Model, -methyl-D-galactopyranoside, HPLC, BBLLectin, bauhinia bauhinioides, caesalpinoideae, pro-inflammatory activity, galactose-specific lectin, proteolytic enzymes, L-NAME, Lectin Purification, Molecular Mass Determination, Electrospray ionization, mass spectrometry (ESI-MS), bovine serum albumin (BSA), Rat Paw Edema Model, -methyl-D-galactopyranoside, HPLC, BBL

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Article Details

VOLUME: 18
ISSUE: 4
Year: 2011
Page: [396 - 402]
Pages: 7
DOI: 10.2174/092986611794653987
Price: $58