Letters in Drug Design & Discovery

G. Perry
University of Texas
San Antonio, TX
Email: lddd@benthamscience.org


Anticancer Activity of Ocimum basilicum and the Effect of Ursolic Acid on the Cytoskeleton of MCF-7 Human Breast Cancer Cells

Author(s): Kehkashan Arshad Qamar, Ahsana Dar, Bina S. Siddiqui, Nurul Kabir, Huma Aslam, Shakil Ahmed, Shaista Erum, Shazia Habib, Sabira Begum.


The anticancer activity of Ocimum basilicum extract and its fractions was evaluated using human cancer cell lines; active compound(s) residing in it were identified and mechanism of their anti-proliferative action was explored. Methanolic extract was fractionated into petroleum ether soluble (PE-S) and insoluble (PE-I) fractions. These were evaluated on HT-144, MCF-7, NCI-H460 and SF-268 cell lines using Sulforhodamine B assay. Immunofluorescence microscopy was employed to study their effects on the cytoskeleton and nuclei of MCF-7 cells. Fractionation of PE-I (GI50: 5 μg/ml; LC50: 71μg/ml against MCF-7) led to the isolation of four compounds, mainly ursolic acid (LC50: 18.6 μg/ml). Ursolic acid (100 μM) induced a significant decrease in the percentage of cells in anaphase/telophase stages along with F-actin aggregation and mitotic spindle distortion. These results support anti-proliferative activity of O. basilicum extract against MCF-7 cells which may partly be due to effects of ursolic acid on F-actin and microtubules.

Keywords: Anticancer, Cytoskeleton, MCF-7 cell line, Ocimum basilicum, Ursolic acid, petroleum ether soluble, PE-S, PE-I, HT-144, MCF-7, NCI-H460, SF-268, Betula alba, paclitaxel, vinblastine, Camptothecin acuminate, Podophyllum pel-tatum, Anvirzel, Nerium oleander, Oleanolic acid, 3-Epi-ursolic acid, 3-O-Methyl ursolic acid, DMSO, DAPI, SRB, trichloroacetic acid, trypan blue, tryp-sin-EDTA, MeOH, EtOAc, HPLC, photodiode array (PDA) detector, RPMI-1640, sulforhodamine B, Immunofluorescence Microscopy, Mitotic Index Assay, ANOVA, FITC channel, XTT assay, Jasplakinolide

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Article Details

Year: 2010
Page: [726 - 736]
Pages: 11
DOI: 10.2174/1570180811007010726
Price: $58