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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus

Author(s): Alessia Ruggiero, Flavia Squeglia, Viviana Izzo, Alba Silipo, Luigi Vitagliano, Antonio Molinaro and Rita Berisio

Volume 17, Issue 10, 2010

Page: [1296 - 1299] Pages: 4

DOI: 10.2174/092986610792231401

Price: $65

Abstract

PrkC is an important Ser/Thr membrane kinase of Staphylococcus aureus able to bind peptidoglycans through extra-cellular domains, denominated as PASTA. Upon peptidoglycan binding, PrkC is activated and stimulates bacterial growth and revival from latency. The entire extra-cellular region of PrkC (residues 378-664), containing three predicted PASTA domains and an extra-domain of unknown function, has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and refinement is in progress.

Keywords: Crystal, cell wall, X-ray, latency


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