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Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
VOLUME: 17
ISSUE: 10
DOI: 10.2174/092986610792231401      Price:  $58









Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Peptidoglycan Binding Region of the Ser/Thr Kinase PrkC from Staphylococcus aureus

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Author(s): Alessia Ruggiero, Flavia Squeglia, Viviana Izzo, Alba Silipo, Luigi Vitagliano, Antonio Molinaro and Rita Berisio
Pages 1296-1299 (4)
Abstract:
PrkC is an important Ser/Thr membrane kinase of Staphylococcus aureus able to bind peptidoglycans through extra-cellular domains, denominated as PASTA. Upon peptidoglycan binding, PrkC is activated and stimulates bacterial growth and revival from latency. The entire extra-cellular region of PrkC (residues 378-664), containing three predicted PASTA domains and an extra-domain of unknown function, has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and refinement is in progress.
Keywords:
Crystal, cell wall, X-ray, latency
Affiliation:
Via Mezzocannone, n. 16. I-80134 Napoli, Italy.