Aspartimide Modified Galanin Analogue Antagonizes Galanin Action on Insulin Secretion

Author(s): Jaroslaw Ruczynski, Zdzislaw Konstanski, Magdalena Cybal, Ivan Kocic, Piotr Rekowski.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 10 , 2010

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Abstract:

Aspartimide (Asi) formation is one of the most serious side reactions that can occur both during solid phase synthesis and storage of peptides containing aspartic acid. Although numerous studies on the mechanism of Asi formation conducted so far, the problem still remains unresolved and relatively little is known about the impact of this side reaction on biological properties of such modified peptides. In the present work we characterized the effect of Asi formation on biological properties of galanin(1-15) analogue modified in position 14 with aspartic acid, investigating its action on rat isolated gastric smooth muscles and glucose-induced insulin secretion from rat isolated islets of Langerhans. Our results show that this side process may adversely affect biological properties of such modified peptides. As we expected, modification of GAL(1-15)NH2 structure changed the interaction of GAL(1-15)NH2 with its receptors and consequently yielded peptide which, in studies on insulin secretion, showed insulinotropic- and antagonistic activities as compared to Asi-free analogue.

Keywords: Aspartimide, galanin analogue, insulin secretion, smooth muscle

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Article Details

VOLUME: 17
ISSUE: 10
Year: 2010
Page: [1182 - 1188]
Pages: 7
DOI: 10.2174/092986610792231447
Price: $65

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