Structural Disorder in Proteins of the Rhabdoviridae Replication Complex
Cedric Leyrat, Francine C.A. Gerard, Euripedes de Almeida Ribeiro Jr., Ivan Ivanov, Rob W.H. Ruigrok and Marc Jamin
Affiliation: Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6, rue Jules Horowitz, B.P. 181, 38042 Grenoble Cedex 9, France.
Rhabdoviridae are single stranded negative sense RNA viruses. The viral RNA condensed by the nucleoprotein (N), the phosphoprotein (P) and the large subunit (L) of the RNA-dependent RNA polymerase are the viral components of the transcription/replication machineries. Both P and N contain intrinsically disordered regions (IDRs) that play different roles in the virus life cycle. Here, we describe the modular organization of P based on recent structural, biophysical and bioinformatics data. We show how flexible loops in N participate in the attachment of P to the N-RNA template by an induced- fit mechanism. Finally, we discuss the roles of IDRs in the mechanism of replication/transcription, and propose a new model for the interaction of the L subunit with its N-RNA template.
Keywords: Rhabdoviridae, replication complex, phosphoprotein, nucleoprotein, intrinsically disordered regions
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