Login

Journal Image
Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
VOLUME: 17
ISSUE: 8
DOI: 10.2174/092986610791498939      Price:  $58









Structural Disorder in Proteins of the Rhabdoviridae Replication Complex

img
Author(s): Cedric Leyrat, Francine C.A. Gerard, Euripedes de Almeida Ribeiro Jr., Ivan Ivanov, Rob W.H. Ruigrok and Marc Jamin
Pages 979-987 (9)
Abstract:
Rhabdoviridae are single stranded negative sense RNA viruses. The viral RNA condensed by the nucleoprotein (N), the phosphoprotein (P) and the large subunit (L) of the RNA-dependent RNA polymerase are the viral components of the transcription/replication machineries. Both P and N contain intrinsically disordered regions (IDRs) that play different roles in the virus life cycle. Here, we describe the modular organization of P based on recent structural, biophysical and bioinformatics data. We show how flexible loops in N participate in the attachment of P to the N-RNA template by an induced- fit mechanism. Finally, we discuss the roles of IDRs in the mechanism of replication/transcription, and propose a new model for the interaction of the L subunit with its N-RNA template.
Keywords:
Rhabdoviridae, replication complex, phosphoprotein, nucleoprotein, intrinsically disordered regions
Affiliation:
Unit of Virus Host Cell Interactions (UVHCI), UMI 3265 UJF-EMBL-CNRS, 6, rue Jules Horowitz, B.P. 181, 38042 Grenoble Cedex 9, France.