Tetrac-Conjugated Quantum Dots for Diagnosis and Treatment of Pancreatic Cancer: An In Vitro Evaluation

Author(s): Dhruba J. Bharali, Usawadee Dier, Paul J. Davis, Shaker A. Mousa.

Journal Name: Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry (Under Re-organization)
(Formerly Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents)

Volume 9 , Issue 4 , 2009

Abstract:

We report a preliminary investigation of the use of pegylated quantum dots (PEG-QDs) conjugated to tetraiodothyroacetic acid (tetrac), a thyroid hormone antagonist and anti-proliferative agent, as a luminescent probe for live cell imaging and treatment of human pancreatic cancer. PEG-QDs were conjugated to epoxyactivated tetrac through primary amino groups present on the PEG-QDs. Cellular entry of tetrac-conjugated PEG-QDs (tetrac-PEG-QDs) in vitro was examined by confocal microscopy and effects of PEG-QDs on cell proliferation were examined by CyQUANT® Cell Proliferation Assay. Tetrac-PEG-QDs exhibited a higher level of entry into pancreatic cancer (PANC-1) cells than unconjugated PEG-QDs. In addition, pre-incubation of cells with the thyroid hormone analogue L-thyroxine (T4) increased fluorescent intensity, indicating enhanced uptake of tetrac-PEG-QDs. Proliferation of PANC-1 cells was significantly inhibited by treatment with tetrac-PEG-QDs.

Keywords: Quantum dots, tetrac, pancreatic cancer, confocal microscopy, cell proliferation, PANC-1 cells

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Article Details

VOLUME: 9
ISSUE: 4
Year: 2009
Page: [219 - 223]
Pages: 5
DOI: 10.2174/187152209790773048
Price: $58

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