Stationary Phase Expression, Purification, and Characterization of XorKI, a Restriction Endonuclease from Xanthomonas oryzae pv. oryzae

Author(s): Woo Young Kang, Young Kee Chae.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 3 , 2010

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Abstract:

An endonuclease from Xanthomonas oryzae pathovar oryzae KACC 10331, XorKI, was heterologously produced in Escherichia coli by applying the stationary state induction method. The yield was 5.4 mg of XorKI per liter of LB medium. XorKI existed in multiple oligomeric forms as evidenced by gel filtration chromatography. The specific activity of purified XorKI was 323000 units per mg.

Keywords: XorKI, restriction endonuclease, Xanthomonas, stationary state induction

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Article Details

VOLUME: 17
ISSUE: 3
Year: 2010
Page: [381 - 385]
Pages: 5
DOI: 10.2174/092986610790780378
Price: $65

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