Mutagenesis Studies of Human Cystathionine β-Synthase: Residues Important for Heme Binding and Substrate Interactions

Author(s): Shin-ichi Ozaki, Chihori Sakaguchi, Akira Nakahara, Masahiro Yoshiya.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 3 , 2010

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Abstract:

Human cystathionine β-synthase (CBS) is a pyridoxal 5-phosphate (PLP) dependent hemoprotein, which catalyzes the condensation of serine and homocysteine. Our mutagenesis studies suggest that Arg-266 is important to sense structural changes in heme-binding site, and that Gln-222 as well as Tyr-223 are involved in interactions with substrates.

Keywords: Cystathionine, hydrogen sulfide, heme, pyridoxal 5'-phosphate

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Article Details

VOLUME: 17
ISSUE: 3
Year: 2010
Page: [351 - 355]
Pages: 5
DOI: 10.2174/092986610790780233
Price: $65

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