Characterization of a New Defensin from Cowpea (Vigna unguiculata (L.) Walp.)

Author(s): Lara Padovan, Ludovica Segat, Alessandro Tossi, Tercilio Calsa Jr., Akio Kido Ederson, Lucas Brandao, Rafael L. Guimaraes, Valesca Pandolfi, Maria Clara Pestana-Calsa, Luis Carlos Belarmino, Ana Maria Benko-Iseppon, Sergio Crovella.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 3 , 2010

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Abstract:

Using Phaseoleae defensins available in databases, a putative defensin gene was isolated in cowpea (Vigna unguiculata (L.) Walp.) and cloned from genomic cowpea DNA. The putative mature defensin sequence displays the characteristic defensins residues arrangement, secondary and tertiary structures were predicted and splicing analysis was performed. Using RT-PCR, defensin expression and differences in response to biotic stimuli between infected and non infected plants were tested.

Keywords: Antimicrobial peptides, cowpea mosaic virus, evolution, plant defensin, protein function, Vigna unguiculata

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Article Details

VOLUME: 17
ISSUE: 3
Year: 2010
Page: [297 - 304]
Pages: 8
DOI: 10.2174/092986610790780350
Price: $65

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