Evaluation of Synthetic Cell-Penetrating Peptides, Pro-Rich Peptide and Octaargine Derivatives, as Adenovirus Vector Carrier

Author(s): Shinya Kida, Yusuke Eto, Yasuo Yoshioka, Shinsaku Nakagawa, Koichi Kawasaki, Mitsuko Maeda.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 2 , 2010

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Abstract:

Two cell-penetrating peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)3-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.

Keywords: Adenovirus, Pro-rich peptide, octaarginine, adenovirus vector, peptide synthesis, cell-penetrating peptide

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2010
Page: [164 - 167]
Pages: 4
DOI: 10.2174/092986610790225978
Price: $58

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