Identification of the Amino Acid Residues Affecting the Catalytic Pocket of the Sulfolobus solfataricus Signature Amidase

Author(s): Cilia Elisa, Ammendola Sergio.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 2 , 2010

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Abstract:

36 mutants of the Sulfolobus solfataricus amidase were analyzed by comparing biochemical data to structural data obtained by a learning machine. The analysis shows that beside well known catalytic residues, amino acid residues Arg197, Lys209 and Asp228 are important for the catalytic activity of the signature thermophilic amidase.

Keywords: Functional residues, amidase, machine learning, SVM, FRAP

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Article Details

VOLUME: 17
ISSUE: 2
Year: 2010
Page: [146 - 150]
Pages: 5
DOI: 10.2174/092986610790226021
Price: $58

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