Expression, Purification, Crystallization and Preliminary X-Ray Crystallographic Analysis of the Resuscitation Promoting Factor Interacting Protein RipA from M. tuberculosis

Author(s): Alessia Ruggiero, Flavia Squeglia, Carla Esposito, Daniela Marasco, Emilia Pedone, Carlo Pedone, Rita Berisio.

Journal Name: Protein & Peptide Letters

Volume 17 , Issue 1 , 2010

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Abstract:

RipA is an important growth factor of M. tuberculosis. Its depletion produces decreasing bacterial growth and abnormal phenotype. RipA C-terminal fragment (263-472), containing its predicted catalytic domain has been successfully crystallized using vapor-diffusion methods. The structure has been solved by Multiwavelength Anomalous Dispersion and atomic resolution refinement is in progress.

Keywords: Resuscitation promoting factor, crystal, growth factor, x-ray, tuberculosis

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Article Details

VOLUME: 17
ISSUE: 1
Year: 2010
Page: [70 - 73]
Pages: 4
DOI: 10.2174/092986610789909557

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