Identifying Sequences Potentially Related to Resistance Response of Piper tuberculatum to Fusarium solani f. sp. piperis by Suppression Subtractive Hybridization

Author(s): Soelange Bezerra Nascimento, Julio Cezar de Mattos Cascardo, Ilmarina Campos de Menezes, Maria de Lourdes Reis Duarte, Sylvain Darnet, Maria Lucia Harada, Claudia Regina Batista de Souza.

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 12 , 2009

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Abstract:

Piper tuberculatum is an exotic Piper from the Amazon region that shows resistance to infection by Fusarium solani f. sp. piperis, causal agent of Fusarium disease in black pepper (Piper nigrum L.). In this work we aimed to study the interaction between P. tuberculatum and F. solani f. sp. piperis at a molecular level, using suppression subtractive hybridization to identify genes potentially related to Fusarium disease resistance. Comparative sequence analysis confirmed that clones isolated here show a high identity with genes coding for proteins that have a known role in plant defense response mechanisms, such as peroxidase, hydroxyproline-rich glycoprotein and CBL-interacting protein kinase. The present study constitutes the first effort to understand the molecular basis of this plant-pathogen interaction, identifying genes which may be used in the future genetic improvement of black pepper.

Keywords: Black pepper, Fusarium disease, Hydroxyproline-rich glycoprotein, Peroxidase, Piper tuberculatum - Fusarium solani f. sp. piperis interaction, Subtractive library

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Article Details

VOLUME: 16
ISSUE: 12
Year: 2009
Page: [1429 - 1434]
Pages: 6
DOI: 10.2174/092986609789839368
Price: $58

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