FCS-Based Sensing for the Detection of Ochratoxin and Neomycin in Food

Author(s): Antonio Varriale, Maria Staiano, Luisa Iozzino, Lorella Severino, Aniello Anastasio, Maria Luisa Cortesi, Sabato D'Auria.

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 12 , 2009

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In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.

Keywords: Food safety, Micotoxins, FCS, Biosensors, Antibodies

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Article Details

Year: 2009
Page: [1425 - 1428]
Pages: 4
DOI: 10.2174/092986609789839359
Price: $58

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