Novel Antimicrobial Peptides Isolated from Skin Secretions of the Mexican Frog Hyla eximia

Author(s): Oscar Villa-Hernandez , Lorena Hernandez-Orihuela , Maria del Carmen Rodriguez , Fernando Zamudio-Zuniga , Ruben Castro-Franco , Victoria Pando , Cesar V.F. Batista .

Journal Name: Protein & Peptide Letters

Volume 16 , Issue 11 , 2009

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Abstract:

In this work, we describe the original characterization of peptides and proteins present in the skin secretions of the Mexican amphibian Hyla eximia. To this purpose, a novel water/dark extraction method, as well as the classic electrical stimulation procedure, was applied in order to extract the skin secretion. Two novel antimicrobial peptides He-1 and He-2 were sequenced. In addition, a molecular mass fingerprint revealed more than one hundred different molecules. Eight peptides in homogeneous form were assayed against five species of bacteria. Thereafter, the peptide He-2 demonstrated high antiparasitic activity against ookinete forms of malaria parasites at low concentration.

Keywords: Hyla eximia, antimicrobial peptides, mass spectrometry

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Article Details

VOLUME: 16
ISSUE: 11
Year: 2009
Page: [1371 - 1378]
Pages: 8
DOI: 10.2174/092986609789353727
Price: $58

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