Azaphilone α-Bromoacetates (AzαBs): Fluorescent Linchpin Reagents for the Inter- and Intramolecular Cross-Linkage of Primary Amines to Thiols
Jason J. Chruma, Sung-Ju Moon and William E. Sanford Jr.
Pages 367-371 (5)
α-Bromoacetate esters of the azaphilone isochromene-6,8-dione core exhibit a pH-dependent orthogonal reactivity allowing for the one-pot cross-linkage of primary amines to thiols in an inter- or intramolecular fashion to afford fluorescent products. This represents a new potential strategy for bioconjugate chemistry.
Azaphilones, bioconjugation, orthogonal reactivity, cross-linkage, fluorescent
University of Virginia, Department of Chemistry, P.O. BOX 400319, Charlottesville, VA 22904- 4319, USA.