Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli
Gareth J. Morrison,
Emily S. Seo,
David J. Clarke,
Helen L. Fielder,
Julia R. Dorin,
John R. W. Govan,
C. Logan Mackay,
Dominic J. Campopiano.
Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 β-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.
Keywords: HBD2, Defensin, Peptide, E. coli, Chemotaxis, Antimicrobial activity
Rights & PermissionsPrintExport