Protein & Peptide Letters

Prof. Ben M. Dunn  
Department of Biochemistry and Molecular Biology
University of Florida
College of Medicine
P.O. Box 100245
Gainesville, FL
USA
Email: bdunn@ufl.edu

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Efficient Production of Human β-Defensin 2 (HBD2) in Escherichia coli

Author(s): Thomas Vargues, Gareth J. Morrison, Emily S. Seo, David J. Clarke, Helen L. Fielder, Julien Bennani, Uday Pathania, Fiona Kilanowski, Julia R. Dorin, John R. W. Govan, C. Logan Mackay, Dusan Uhrin, Dominic J. Campopiano.

Abstract:

Human β-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 β-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Keywords: HBD2, Defensin, Peptide, E. coli, Chemotaxis, Antimicrobial activity

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Article Details

VOLUME: 16
ISSUE: 6
Year: 2009
Page: [668 - 676]
Pages: 9
DOI: 10.2174/092986609788490122
Price: $58