Title: Reduced NGF Secretion by HT-29 Human Colon Cancer Cells Treated with a GRPR Antagonist
VOLUME: 16 ISSUE: 6
Author(s):Caroline Brunetto de Farias, Laura Stertz, Rodrigo Cruz Lima, Flavio Kapczinski, Gilberto Schwartsmann and Rafael Roesler
Affiliation:Department of Pharmacology, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite, 500 (ICBS, Campus Centro/UFRGS), 90046-900 Porto Alegre, RS (Brazil).
Keywords:Gastrin-releasing peptide receptor, RC-3095, nerve growth factor, colon cancer
Abstract: The gastrin-releasing peptide receptor (GRPR) is a therapeutic target in colon cancer. Here we show that the GRPR antagonist RC-3095 (10 , 10 , or 1 μM) decreases nerve growth factor (NGF) secretion measured by enzymelinked immunosorbent assay (ELISA) in HT-29 human colon carcinoma cells. The results suggest that decreased secretion of neurotrophins might be a novel mechanism by which GRPR antagonists exert their antiproliferative effects in cancer cells.
