Application of GFP-Labeling to Study Prions in Yeast
Lois E. Greene, Yang-Nim Park, Daniel C. Masison and Evan Eisenberg
Affiliation: Laboratory of Cell Biology, NHLBI, 50 South Dr Rm 2537 MSC 8017, Bethesda, MD 20892-8017, USA.
Fluorescent live cell imaging has recently been used in numerous studies to examine prions in yeast. These fluorescence studies take advantage of the fact that unlike the normally folded form, the misfolded amyloid form of the prion protein is aggregated. The studies have used fluorescence to identify new prions, to study the transmission of prion from mother to daughter, and to understand the role of molecular chaperones in this transmission. The use of fluorescence imaging complements the more standard methods used to study prion propagation. This review discusses the various studies that have taken advantage of fluorescence imaging technique particularly in regard to understanding the transmission and curing of the [PSI ], the prion form of the translation termination factor Sup35p.
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