Amino acids and some of their derivatives are strong polar organic compounds. Non-deuterated formic acid was used as an inexpensive, convenient, and practical 13C NMR solvent for these strong polar compounds. It was also used to determine hydroxyl carboxylic acids and nucleosides. 13C NMR chemical shifts in formic acid of representative examples and widely used solvents in organic reactions were provided. Formic acid is a counterpart to commonly used perdeuterated chloroform, dimethyl sulfoxide, methanol, and water.
Keywords: Amino acid, Carbon-13 nuclear magnetic resonance spectrum, Peptide, N-protected amino acid, Polar organic compound, Solvent
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