Analysis of Pralidoxime in Serum, Brain and CSF of Rats

Author(s): Huba Kalasz , Eva Szoko , Tamas Tabi , Georg A. Petroianu , Dietrich E. Lorke , Abdulrab Omar , Salem Alafifi , Almerri Jasem , Kornelia Tekes .

Journal Name: Medicinal Chemistry

Volume 5 , Issue 3 , 2009

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Abstract:

After administration of various amounts of pralidoxime to rats, the levels in serum, brain and cerebrospinal fluid (CSF) were measured using capillary zone electrophoresis (CZE). The calibration curves were established using spiked samples. The calibration covers the ranges from 0.3 – 200 μg/mL, 0.3 – 7 μg/mL and 0.1 – 7 μg/mL for serum, brain and CSF, respectively. The CZE measurement opens the way to the fast and reliable determination of pyridinium aldoxime concentrations in serum, cerebrospinal fluid and brain, thereby monitoring blood-brain and blood-CSF penetration of pyridinium aldoximetype antidotes clinically used in organophosphate poisoning.

Keywords: CZE, pralidoxime, serum, cerebrospinal fluid (CSF), brain

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Article Details

VOLUME: 5
ISSUE: 3
Year: 2009
Page: [237 - 241]
Pages: 5
DOI: 10.2174/157340609788185882
Price: $58

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