Ca(II) -γ-Thionin complex: Interaction Studies by Differential Pulse Voltammetry and MALDI-TOF / MS

Author(s): Clarissa Silva Pires de Castro, Jurandir Rodrigues DeSouza, Carlos Bloch Junior.

Journal Name: Protein & Peptide Letters

Volume 9 , Issue 1 , 2002

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Abstract:

In this work we investigated the formation of a complex between γ-thionin SIα1 and Ca2+ using differential pulse voltammetry and MALDI-TOF / MS. A stoichiometry rate of one calcium ion per one SIα1 molecule was obtained. Kd values of 1.31 x 10-9 mol L-1, 2.63 x 10-8 mol L-1 and 2.71 x 10 -8 mol L-1 were determined for the Ca2+-SIα1 complex by three different methodologies. These findings contribute to a better understanding of the SIα1 inhibition mechanism towards insect and mammalian α-amylases.

Keywords: THIONIN COMPLEX, cell-free systems, Matrix-assisted laser, amperometric titration

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Article Details

VOLUME: 9
ISSUE: 1
Year: 2002
Page: [45 - 52]
Pages: 8
DOI: 10.2174/0929866023409020

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